Language
RayBio®线粒体DNA分离试剂盒可用于从各种细胞和组织中分离mtDNA（12个样品1.5小时），产量高，纯度高，没有核基因组DNA污染。
纯化的mtDNA可用于后续各种研究，如酶处理，southern blotting，克隆，PCR分析和扩增。
货号：MT-ISO
规格：50T/100T
适用样本：兼容各种细胞、组织······
产品特性：无核DNA污染、mtDNA产量高，纯度高
Kit Components
| Component | Size / Description |
|---|---|
| Cell Lysis Bufer-1 | 30 mL/bottle |
| Cell Lysis Bufer-2 | 3 mL/bottle |
| mtDNA Lysis Buffer | 30 mL/bottle |
| Protease K | 0.4 mL/vial |
| Magnetic Bead Solution | 0.3 mL/vial |
| Binding Buffer | 30 mL/bottle |
| Wash Buffer A | 30 mL/bottle |
| Wash Buffer B | 30 mL/bottle |
| Wash Buffer C | 30 mL/bottle |
| Elution Buffer | 3 mL/bottle |
Additional Materials Required
- Sterile nuclease-free pipette tips (barrier tips recommended) and microfuge tubes
- 1X PBS buffer
- Microcentrifuge
- Magnetic Separator for Microcentrifuge Tubes (2 mL)
- Vortex Mixer
- Water bath or dry heat block
- 100% ethanol (30 mL)
- Precision pipettes to deliver 2 L to 1 mL volumes
- Tissue homogenizer (Dounce tissue grinder) for tissue samples
- Refrigerated centrifuge with swing out rotor and refrigerated microcentrifuge
Assay Procedure Summary
- Prepare cell or tissue samples as instructed.
- Cell lysis with Cell Lysis Bufer-1 for 15 min and Cell Lysis Bufer-2 for another 2 minutes.
- mtDNA lysis with Protease K and mtDNA Lysis Buffer at 56oC for 10 minutes.
- mtDNA binding with the magnetic beads at RT for 10 minutes.
- mtDNA washing with Wash Buffer A, B and C.
- mtDNA elution with elution buffer.
- mtDNA can be used or stored.