Product Feature

High density arrays
1000 Human proteins
Direct biotin labeling of proteins
High detection sensitivity
Accurate and reproducible
Affordable, quick and simple to use

Target Name

11b-HSD1, 2B4, 4-1BB, 6Ckine, A1BG, A2M, ABL1, ACE, ACE-2, ACK1, ACPP, ACTH, Activin A, Activin B, Activin C, Activin RIA / ALK-2, Activin RIB / ALK-4, Activin RII A/B, Activin RIIA, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4 , ADAMTS-5, ADAMTS-L2, Adiponectin / Acrp30, Adipsin, Afamin, AFP, AgRP, ALBUMIN, ALCAM, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha 1 AG, Alpha 1 Microglobulin, Alpha Lactalbumin, ALPP, AMICA, AMPKa1, Amylin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, ANGPTL3, ANGPTL4, Annexin A7, APC, APCS, Apelin, Apex1, APJ, APN, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APP, APRIL, AR (Amphiregulin), Artemin, ASPH, Attractin, Axl, B3GNT1, B7-1 /CD80, BAF57, BAFF, BAFF R / TNFRSF13C, BAI-1, BCAM, BCMA / TNFRSF17, BD-1, BDNF, Beta 2M, Beta Defensin 4, Beta IG-H3, beta-Catenin, beta-Defensin 2, beta-NGF, Biglycan, BIK, BLAME, BLC / BCA-1 / CXCL13, BMP-15, BMP-2, BMP-3, BMP-3b / GDF-10, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-9, BMPR-IA / ALK-3, BMPR-IB / ALK-6, BMPR-II, BMX, BNIP2, BNP, BTC, Btk, C2, C3a, C5/C5a, C7, C8B, C9, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, Cardiotrophin-1 / CT-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CCL14 / HCC-1 / HCC-3, CCL28 / VIC, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD 163, CD14, CD200, CD23, CD24, CD27 / TNFRSF7, CD30 / TNFRSF8, CD30 Ligand / TNFSF8, CD36, CD38, CD40 / TNFRSF5, CD40 Ligand / TNFSF5 /CD154, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Cerberus 1, Ceruloplasmin, CFHR2, Chem R23, Chemerin, CHI3L1, Chordin-Like 1, Chordin-Like 2, Chromogranin A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLC, CLEC3B, Clusterin, CNDP1, CNTF R alpha, CNTF, Coagulation Factor III / Tissue Factor, COCO, Complement factor H, Contactin-1, Contactin-2, Corticosteroid-binding globulin, COX-2, C-peptide, CPN2, Creatinine, CRIM 1, Cripto-1, CRP, CRTAM, CRTH-2, Cryptic, CSH1, Csk, CTACK / CCL27, CTGF / CCN2, CTLA-4 /CD152, cTnT / Troponin T, CutA, CV-2 / Crossveinless-2, CXCL14 / BRAK, CXCL16, CXCR1 / IL-8 RA, CXCR2 / IL-8 RB, CXCR3, CXCR4 (fusin), CXCR5 /BLR-1, CXCR6, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 8, Cytokeratin18, Cytokeratin19, D6, DAN, DANCE, DBI, DCBLD2, DcR3 / TNFRSF6B, D-Dimer, Decorin, DEFA1/3, Defensin, Desmin, Dkk-1, Dkk-3, Dkk-4, DLL1, DLL4, DMP-1, DPPIV, DR3 / TNFRSF25, DR6 / TNFRSF21, Dtk, E-Cadherin, EDA-A2, EDAR, EDG-1, EGF, EGF R / ErbB1, EG-VEGF / PK1, EMAP-II, ENA-78, Endocan, Endoglin / CD105, Endorphin Beta, Endostatin, Endothelin, Endothelin Receptor A, Enolase 2, ENPP2, EN-RAGE, Eotaxin / CCL11, Eotaxin-2 / MPIF-2, Eotaxin-3 / CCL26, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, Epiregulin, ErbB2, ErbB3, ErbB4, ERRa, Erythropoietin R, Erythropoietin, ESAM, E-Selectin, EV15L, EXTL2, FABP1, FABP2, FABP3, FABP4, Factor XIII A, Factor XIII B, FADD, FAK, FAM3B, FAP, Fas / TNFRSF6, Fas Ligand, Fc RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGF Basic, FGF R3, FGF R4, FGF R5, FGF-10 / KGF-2, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-6, FGF-7 / KGF, FGF-8, FGF-9, FGF-BP, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, FOLR1, FOXN3, FoxO1, FoxP3, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, FRK, FSH, Furin, Fyn, GADD45A, Galanin, Galectin-1, Galectin-3, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas1, GASP-1 / WFIKKNRP, GASP-2 / WFIKKN, Gastrin, GATA-3, GATA-4, GCP-2 / CXCL6, GCSF, G-CSF R / CD 114, GDF1, GDF11, GDF-15, GDF3, GDF5, GDF8, GDF9, GDNF, Gelsolin, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, Ghrelin, GITR / TNFRF18, GITR Ligand / TNFSF18, GLP-1, Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), GRP, GRP75, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, HB-EGF, HCC-4 / CCL16, hCG alpha, hCGb, Hck, HCR / CRAM-A/B, HE4, Hemopexin, Hepassocin, Hepcidin, Heregulin / NDF / GGF / Neuregulin, HGF, HGFR, HOXA10, HRG-alpha, HRG-beta 1, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, HSPA8, HTRA2, HVEM / TNFRSF14, I-309, IBSP, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha / beta R1, IFN-alpha / beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGF2BP1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGFBP-rp1 / IGFBP-7, IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F10 / IL-1HY2, IL-1 F5 / FIL1delta, IL-1 F6 / FIL1 epsilon, IL-1 F7 / FIL1 zeta, IL-1 F8 / FIL1 eta, IL-1 F9 / IL-1 H1, IL-1 R3 / IL-1 R AcP, IL-1 R4 /ST2, IL-1 R6 / IL-1 Rrp2, IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha /IL-1 R5, IL-18 R beta /AcPL, IL-19, IL-2, IL-2 R alpha, IL-2 R beta /CD122, IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-23p19, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-3, IL-3 R alpha, IL-31, IL-31 RA, IL-33, IL-34, IL36RN, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9 , Inhibin A, Inhibin B, INSL3, INSRR, Insulin, Insulin R, Insulysin / IDE, Integrin alpha V, IP-10, I-TAC / CXCL11, Itk, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, Kininostatin / kininogen, KLF4, Kremen-1, Kremen-2, LAG-3, Latent TGF-beta bp1, Layilin, LBP, Lck, LDL R, LECT2, Lefty - A, Legumain, Leptin (OB), Leptin R, LFA-1 alpha, LH, LIF R alpha, LIF, LIGHT / TNFSF14, LIMPII, LIN41, Lipocalin-1, Livin, LOX-1, LPS, LRG1, LRP-1, LRP-6, L-Selectin (CD62L), LTF, LTK, Luciferase, Lumican, Lymphotactin / XCL1, Lymphotoxin beta / TNFSF3, Lymphotoxin beta R / TNFRSF3, Lyn, LYRIC, LYVE-1, LZTS1, MAC-1, Mammaglobin A, Marapsin, MATK, MBL, MBL-2, MCP-1, MCP-2, MCP-3, MCP-4 / CCL13, M-CSF, M-CSF R, MDC, Mer, Mesothelin, MFG-E8, MFRP, MICB, Midkine, MIF, MIG, MINA, MIP 2, MIP-1a, MIP-1b, MIP-1d, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-10, MMP-11 /Stromelysin-3, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 / MT3-MMP, MMP-19, MMP-2, MMP-20, MMP-24 / MT5-MMP, MMP-25 / MT6-MMP, MMP-3, MMP-7, MMP-8, MMP-9, MSHa, MSP alpha Chain, MSP beta-chain, MTUS1, Musk, Myoglobin, NAIP, Nanog, NAP-2, NCAM-1 / CD56, NELL2, NEP, Nesfatin, Nestin, NET1, Netrin G2, Netrin-4, Neuritin, NeuroD1, Neurokinin-A, Neuropeptide Y, Neuropilin-2, Neurturin, NF1, NGF R, NM23-H1/H2, Notch-1, NOV / CCN3, NPTX1, NPTXR, NR3C3, NRG1 Isoform GGF2, NRG1-alpha / HRG1-alpha, NRG1-beta1 / HRG1-beta1, NRG2, NRG3, NT-3, NT-4, Ntn1, OCT3/4, Omentin, Orexin A, Orexin B, OSM, Osteoactivin / GPNMB, Osteocalcin, Osteocrin, Osteopontin, Osteoprotegerin / TNFRSF11B, OX40, OX40 Ligand / TNFSF4, p21, p27, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, Pappalysin-1, PARC / CCL18, PARK7, P-Cadherin, PCAF, PD-1, PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PDX-1, PECAM-1 /CD31, PEDF , Pentraxin3 / TSG-14, PEPSINOGEN I, PEPSINOGEN II, Persephin, PF4 / CXCL4, PGRP-S, PI 16, PI 3Kinase p85 beta, PIM2, PKM2, Plasminogen, PlGF, PLUNC, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Pref-1, Presenilin 1, Presenilin 2, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, pro-Glucagon, Progranulin, Prohibitin, Prolactin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-Free, PSA-total, P-selectin, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, RAGE, RANK / TNFRSF11A, RANTES, Ras, RBP4, RECK, RELM alpha, RELM beta, RELT / TNFRSF19L, Resistin, RET, RIP1, ROBO4, ROCK1, ROCK2, ROR1, ROR2, ROS, RYK, S100 A8/A9, S100A10, S100A4, S100A6, S100A8, S-100b, SAA, SART1, SART3, SCF, SCF R /CD117, SCG3, SDF-1 / CXCL12, Selenoprotein P, SEMA3A, Serotonin, Serpin 1, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin I1, SERTAD2, sFRP-1, sFRP-3, sFRP-4, sgp130, SHBG, SIGIRR, Siglec-5/CD170, Siglec-9, SLPI, SMAC, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, SMDF / NRG1Isoform, SNCG, Soggy-1, Somatotropin, Sonic Hedgehog (Shh N-terminal), SOST, SOX17, SOX2, SPARC, SPARCL1, Spinesin, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TACI / TNFRSF13B, TAF4, Tarc, TCCR / WSX-1, Tec, TECK / CCL25, TFF1, TFF3, TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI / ALK-5, TGF-beta RII, TGF-beta RIIb, TGF-beta RIII, Thrombin, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin (TSP), Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymidine Kinase-1, Thymopoietin, Thyroglobulin, Tie-1, Tie-2, TIM-1, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A / TNFSF15, TLR1, TLR2, TLR3, TLR4, TMEFF1 / Tomoregulin-1, TMEFF2, TNF RI / TNFRSF1A, TNF RII / TNFRSF1B, TNF-alpha, TNF-beta, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, TRADD, TRAIL R1 / DR4 / TNFRSF10A, TRAIL R2 / DR5 / TNFRSF10B, TRAIL R3 / TNFRSF10C, TRAIL R4 / TNFRSF10D, TRAIL / TNFSF10, TRANCE, Transferrin, Trappin-2, TREM-1, TRKB, Troponin C, Troponin I, TROY / TNFRSF19, TRPC1, TRPC6, TRPM7, Trypsin 1, TSG-6, TSH, TSLP, TSLP, TWEAK / TNFSF12, TWEAK R / TNFRSF12, TXK, Tyk2, TYRO10, Ubiquitin+1, uPA, uPAR, Uromodulin, Vasopressin, Vasorin, VCAM-1 (CD106), VDUP-1, VE-Cadherin, VEGF, VEGF R1, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI / TNFSF15, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-BP, Vitamin K-dependent protein S, Vitronectin, VWF, WIF-1, Wilms Tumor 1, WISP-1 / CCN4, XEDAR, XIAP, ZAG, ZAP70

Target Species

Human

Species Detected

Human

Application Notes

Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing Kit Components

• RayBio® L-Series Human 507 and 493 Antibody Array Glass Slide(s)
• Dialysis Tubes
• Labeling Reagent
• Stop Solution
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Streptavidin-Conjugated Fluor
• Adhesive Plastic Strips
• Serum Buffer
• Floating Dialysis Rack
• 30 ml Centrifuge Tube

Other Materials Required

• Distilled or de-ionized water
• KCl, NaCl, KH2PO4 and Na2HPO4
• Small plastic or glass containers
• Orbital shaker or oscillating rocker
• Beaker, stir plate and stir bar
• 1 ml tube
• Pipettors, pipette tips and other common lab consumables
• Laser scanner for fluorescence detection (list of compatible scanners available at http://www.raybiotech.com/resources.asp)
• Aluminum foil

Protocol Outline

1. Dialyze samples in PBS
2. Biotinylate Samples
3. Dialyze biotinylated samples using spin column
4. Dry the glass slide
5. Block array surface
6. Incubate with Samples
7. Incubate with Streptavidin-Conjugated Fluor
8. Disassemble the glass slide
9. Scan with a gene microarray laser scanner
10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at ≤ -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at ≤ -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

1000