The IVIS^® Lumina K Series III from Revvity provides a real-time, fast pre-clinical in vivo imaging system enabling acquisition of biologically relevant events within milliseconds. The IVIS Lumina K can perform both quantitative bioluminescence and fluorescence imaging as a standard high signal to noise imager and as a high speed imager. The system includes a highly sensitive EMCCD camera for signal enhancement and the ability to reduce exposure times enabling fast kinetics. From the leading optical imaging platform for in vivo analysis, IVIS systems are supported by a range of practical accessories developed through experience in research laboratories worldwide. The Lumina K offers a light-tight injection port which supporting a syringe injector system enabling real time compound and/or substrate administration. A removable pull-out tray is available to facilitate pre-imaging animal procedures.