人生长激素(hGH)ELISA试剂盒

sufficient for 192 tests, >98% (SDS-PAGE), kit of 1, suitable for ELISA

别名:
ELISA

一般描述

The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g., RNA isolation). Additionally, hGH is a mammalian gene that may contribute to the apparent high stability of the hGH mRNA in most mammalian cells.
Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml.
Human growth hormone (hGH) is specifically detected by the hGH ELISA. It does not cross-react with rat growth hormone. Cross-reactivity with the human homologues TSH, FSH, and LH has not been tested. The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.

hGH Standards

The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.

特异性

The hGH ELISA specifically detecs human growth hormone (hGH). It does not cross-react with rat growth hormone.