Taq DNA Polymerase, dNTPack

5 U/βl

别名: dna amplification, pcr, polymerase, primer extension

General description

Contents
Taq DNA Polymerase, 5 U/μl
PCR Buffer, 10x concentrated, with MgCl₂
PCR Nucleotide Mix

For maximum convenience, select the ready-to-use 2x concentrated PCR Master.

Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.

Taq DNA Polymerase is a highly processive 5′→3′ DNA polymerase that lacks 3′→5′ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.

Application

Achieve the most consistent results in simple, routine PCR by using Roche Applied Science Taq DNA Polymerase, which is held to our rigorous purity and quality standards. The Taq DNA Polymerase, dNTPack, combines our standard preparation (5 U/μl) of recombinant Taq DNA Polymerase with our convenient, ready-to-use PCR Nucleotide Mix. In all other respects, this preparation is identical to our standard preparation and can be used for: • PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Features and Benefits

• Reliable reproducible results: High lot-to-lot consistency.
• No need to test each lot: Taq DNA Polymerase is rigorously tested.
• Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.