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首页 > Biomedical Research > EasySep™ 人CD4+ T细胞分选试剂盒
EasySep™ 人CD4+ T细胞分选试剂盒
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品牌 STEMCELL Technologies货号17952
过期 长期有效
更新 2025-08-12 18:48
 
详细信息
EasySep™
简单快捷的免疫磁珠细胞分选
EasySep™是一种强大的免疫磁珠细胞分选平台,它结合了单克隆抗体的特异性和无柱磁珠分选平台的简便性,能够快速、简单地分离出高纯度的细胞群,且可立即用于下游应用。

通过使用对表面抗原具有特异性的抗体复合物,经标记的细胞可被去除(负选),也可被分选(正选)。抗体复合物可将目标细胞与磁珠相连接。当样本被置于EasySep™磁极中,经标记的细胞在磁场的 作用下将被吸附于试管壁上。未经标记的细胞则可被轻松倾倒或吸取到一个新的试管中,而被磁珠标记的细胞则保留在试管中。

EasySep™用于人细胞
短至8分钟的免疫磁珠细胞分选
EasySep™试剂盒可用于从各种样本来源中分选人细胞,包括:外周血单个核细胞(PBMCs)、全血、白细胞单采术(Leukapheresis Pack)样本、骨髓和脐带血。目标细胞被抗体复合物和磁珠标记,以进行负选或正选。
使用我们新一代的EasySep™细胞分选试剂盒,短至8分钟,即可分选高纯度、未经标记的、功能性的人免疫细胞。

操作流程

实验数据

图1. EasySep™人CD4+ T细胞分选试剂盒(产品号 #17952)
起始样本为人外周血单个核细胞,分选所得细胞中CD4+ T细胞(CD3+ CD4+)的含量通常可达94.8±2.3%(平均值±标准误差;以具活性的单线态细胞设门)。在以上示例中,起始样本和分选后的细胞的纯度分别为39.4%和98.2%。

The EasySep™ Human CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product can be used in place of the EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052) for even faster cell isolations.

Advantages:
• Fast, easy-to-use and column-free
• Up to 97% purity with high recovery
• Untouched, viable cells

Components:
  • EasySep™ Human CD4+ T Cell Isolation Kit (Catalog #17952)
    • EasySep™ Human CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
  • RoboSep™ Human CD4+ T Cell Isolation Kit (Catalog #17952RF)
    • EasySep™ Human CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog 18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)

Cell Type:
T细胞; CD4+T细胞

Species:


Sample Source:
白细胞去除术; PBMC