DNASep™ Cartridge – DNA Separation Column
4.6 mm x 50 mm – For high resolution HPLC ds/ss DNA analysis, sizing, and
Mutation Detection. Load capacity of 2µg.

SKU: DNA-99-3510
 

Description
The DNASep™ – DNA separation column uses alkylated non-porous polystyrene-divinylbenzene
(PS-DVB) copolymer microspheres for high performance nucleic acid separations. These versatile
columns can be used with a variety of HPLC systems.
4.6 x 50 mm, non-porous PSDVB resin matrix, column capacity 2.0 μg total DNA

DNA Calibration

Liquid chromatography (LC) is a powerful technique for separation and quantitation of nucleic
acids due to its high-resolving capability, short analysis time, and ease of recovery of DNA fragments
for subsequent studies. Using the DNASep™ column for sizing applications in reversed-phase HPLC
provides many advantages. Fragments can be collected and subjected to further molecular analysis
such as target verification, sub cloning, amplification and sequencing. DNA fragment sizing is required
for quality control (QC) of sequencing templates; quantitation and sizing of DNA fragments; “yes/no”
assays for the presence of a DNA fragment in genotyping. Sizing of dsDNA fragments by ion-pair
reversed-phase HPLC is largely independent of fragment sequence and sequence related secondary
structures of the molecule.