ZymoBIOMICS Microbial Community DNA Standard

Cat #            Name                                                                                       Size

D6305          ZymoBIOMICS Microbial Community DNA Standard         200ng

D6306          ZymoBIOMICS Microbial Community DNA Standard         2000ng

Brand ： Zymo Research 
 

_d6305_d6306_zymobiomics_microbial_community_dna_standard.pdf

 

HIGHLIGHTS

Accurate composition: composition cross-validated with multiple types of measurements.

Negligible impurity: guaranteed to contain < 0.01% foreign microbial DNA.

Wide range of GC content: 15%-85%, for assessing bias caused by GC content variation.

 

DESCRIPTION

One of the major challenges in the emerging field of microbiomics is the bias and errors introduced
in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library
preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is
designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This
DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined
composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content
(15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular
version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when
working in tandem. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 12%,
Pseudomonas aeruginosa - 12%, Bacillus subtilis - 12%, Escherichia coli - 12%,
Salmonella enterica - 12%, Lactobacillus fermentum - 12%, Enterococcus faecalis - 12%,
Staphylococcus aureus - 12%, Saccharomyces cerevisiae - 2%, and Cryptococcus neoformans - 2%.

 

TECHNICAL SRECIFICATIONS

Purity

< 0.01% foreign microbial DNA

Sample Source

A mixture of genomic DNA of ten microbial strains.

Sample Storage

-20 °C

Supplemental Info

How to Quantify Bias with Mock Microbial Community Standards

How to Use Microbial Community Standards