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Zymo Research - D4003 - DNA Cl
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更新 2025-08-12 15:36
 
详细信息

DNA Clean & Concentrator-5

Cat #       Name                                                                    Size

D4004     DNA Clean & Concentrator-5 (Uncapped)        200 Preps

D4003     DNA Clean & Concentrator-5 (Uncapped)        50 Preps

D4014     DNA Clean & Concentrator-5 (Capped)            200 Preps

D4013     DNA Clean & Concentrator-5 (Capped)            50 Preps

D4003T   DNA Clean & Concentrator-5 (Uncapped)        10 Preps

Brand:Zymo Research

 

_d4003t_d4003_d4004_d4013_d4014_dna_clean_concentrator_-5.pdf

 

HIGHLIGHTS

Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash
residue carryover.

Column design allows DNA to be eluted at high concentrations into minimal volumes of water or
TE buffer.

Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion,
RNA transcription, radiolabeling, arrays, etc.

 

DESCRIPTION

The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg
DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling
reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA
polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as
free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is
suitable for PCR, arrays, ligation, sequencing, etc.

 

TECHNICAL SRECIFICATIONS

Detergent Tolerance

≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

Elution Volume

≥ 6 µl

Equipment

Microcentrifuge

Purity

A260/A280 > 1.8

Sample Source

DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling
reactions, etc.

Size Range

50 bp to 23 kb

Yield

≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb
to 23 kb the recovery is 50-70%.