Test procedure: 75 min (20 min Sample Processing + 55 min Thermocycler + 5 min RT)；Evaluation: qualitatively；Standard range: n.a.；Detection limit: 10^3 – 10^4 germs/ml；Sample volume: 2 µl；Specificity: Alicyclobacillus acidoterrestris and ability to form guaiacol；