The Illumina HiSeq 2500 is a high-throughput sequencing system designed for large-scale genomic projects. Here are the key specifications of the HiSeq 2500:

General Specifications:

• Sequencing Technology: Illumina SBS (Sequencing by Synthesis) platform

• Applications: Whole-genome sequencing, exome sequencing, RNA-Seq, targeted sequencing, and more

• Throughput:

  • Up to 1.6 Tb of data per run (HiSeq 2500 High-Output Mode)

  • Up to 600 Gb of data per run (HiSeq 2500 Rapid Mode)

• Read Lengths:

  • 50 bp, 100 bp, 150 bp paired-end reads

  • 250 bp paired-end reads (with software updates)

Throughput Details:

• High-Output Mode:

  • Single Read: Up to 600 Gb of data per run

  • Paired-End Read: Up to 1.6 Tb of data per run

• Rapid Mode:

  • Single Read: Up to 250 Gb of data per run

  • Paired-End Read: Up to 600 Gb of data per run

Instrument Features:

• Dual-Mode Capability:

  • High-Output mode for higher throughput and longer runs (up to 12 days).

  • Rapid Mode for faster runs (typically 1–2 days).

• Flow Cell: Two flow cells with 8 lanes per flow cell

• Cluster Generation: Illumina’s bridge amplification for generating clusters on the flow cell

• Base Calling: Software-driven base calling with Real-Time Analysis (RTA)

Run Time:

• High-Output Mode: Up to 12 days (depending on read length and other settings)

• Rapid Mode: ~1–2 days (depending on read length)

Data Output:

• Base Call Accuracy: >99.99% for Illumina platform

• Maximum Reads per Run: Over 800 million reads (depending on configuration)

Software:

• Real-Time Analysis (RTA): Software for base calling

• Illumina Sequencing Control Software (SCS): Controls the sequencing run process

• BaseSpace Sequence Hub: Optional cloud-based analysis platform

System Requirements:

• Dimensions:

  • Height: 30 inches

  • Width: 29.6 inches

  • Depth: 40 inches

• Weight: Approximately 185 kg (410 lbs)

• Power Consumption: 1,200 W