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BEGM™ Bronchial Epithelial Cell Growth Medium
BEGM BulletKit (CC-3171 & CC-4175) CC-3170
BEBM Basal Medium 500 ml CC-3171
BEGM SingleQuot Kit Suppl. & Growth Factors CC-4175
BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGM™ BulletKit™ is serum free and has been optimized to grow cells from normal and diseased tissues.
Published research shows BEGMTM BulletKit has expanded applications. Our customers have used BEGMTM BulletKitTM to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTM BulletKitTM media may also offer cost-benefit since customers can use the same media to support multiple cell types.
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Data Courtesy of Furukawa, et al. Lonza's NHBE cells, BEAS-2B cell line were co-cultured with breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza's BEGMTM BulletKit Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of lung epithelial cells. Immunofluorescence images of MDA-MB-231 above show breast cancer cells changed morphology when cultured with primary NHBEs and became E-cadherin positive (white arrows). Similar response was not detected with BEAS-2B. The paper highlights the suitability of Lonza's BEGMTM Media for co-culture models. It also illustrates the importance of using Lonza's primary NHBE cells as a side-by-side control when conducting experiments with normal lung cell lines such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines. |
Here are additional publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells:
- El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
- Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza'sHUVEC and NHBE in a 3D environment.
- Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs
For a detailed listing of publications with expanded use of BEGMTM Medium, please contact our scientific support team.
相关产品：
| No. | Cat No. | Description | 库存数量 | 人民币报价 |
| 1 | CC-3162 | EGM-2 BulletKit (CC-3156 & CC-4176) | 100 | ￥3,103.00 |
| 2 | CC-3170 | BEGM BulletKit (CC-3171 & CC-4175) | 50 | ￥3,030.22 |
| 3 | CC-3202 | EGM-2 MV BulletKit (CC-3156 & CC-4147) | 50 | ￥3,078.04 |
| 4 | CC-3132 | FGM-2 BulletKit (CC-3131 & CC-4126) | 25 | ￥2,685.36 |
| 5 | CC-3150 | MEGM BulletKit (CC-3151 & CC-4136) | 25 | ￥2,809.61 |
| 6 | CC-3190 | REGM BulletKit (CC-3191 & CC-4127) | 20 | ￥3,004.86 |
| 7 | CC-3118 | SAGM BulletKit (CC-3119 & CC-4124) | 15 | ￥2,933.86 |
| 8 | CC-3198 | HCM BulletKit (CC-3199 & CC-4182) | 10 | ￥3,522.16 |
| 9 | CC-5035 | EGM-Plus BulletKit (CC-4542 + CC-5036) | 10 | ￥3,689.27 |
| 10 | 04-418Q | X-Vivo 15 w/ Gentamycin and PR, 1 L | 400 | ￥1,265.00 |
| 11 | 12-725F | UltraCULTURE serum-free w/o L-Gln 500ml | 300 | ￥1,092.91 |
| 12 | 12-604F | DMEM 4.5 g/L glucose with L-glutamine, 500 ml | 200 | 买十送一 ¥65/vial |
| 13 | 12-611F | MEM Eagle with Earle's BSS, with L-glutamine, 500 ml | 200 | 买十送一 ¥65/vial |
| 14 | 12-741F | DMEM 4.5 g/L glucose with L-glutamine, without sodium pyruvate, 500 ml | 200 | 买十送一 ¥65/vial |
| 15 | 12-115F | RPMI 1640 with HEPES and L-Glutamine | 200 | 买十送一 ¥65/vial |
| 16 | 17-516F | PBS-1X, w/o Ca++, Mg++ | 100 | 买十送一 ¥65/vial |
| 17 | 17-161E | Trypsin-Versene (EDTA) | 100 | 买十送一 ¥65/vial |
| 18 | 17-605E | L-Glutamine 200mM | 50 | 买十送一 ¥65/vial |
| 19 | 12-702F | RPMI 1640 w/ L-Gln | 50 | 买十送一 ¥65/vial |
| 20 | 12-115F | RPMI 1640 with HEPES and L-Gln | 50 | 买十送一 ¥65/vial |
| 21 | 12-707F | DMEM 1.0 g/L Glucose w/o L-Gln | 50 | 买十送一 ¥65/vial |
| 22 | 12-709F | DMEM 4.5 g/L Glucose w/o L-Gln, w/HEPES | 50 | 买十送一 ¥65/vial |
| 23 | 12-917F | DMEM 4.5g/L Glucose w/o L-Gln, PR, 500ml | 20 | 买十送一 ¥65/vial |
| 24 | 12-719F | DMEM:F12 1:1Mixture with HEPES, L-Gln | 20 | 买十送一 ¥65/vial |
| 25 | 12-719F | DMEM:F12 1:1Mixture with HEPES, L-Gln | 20 | 买十送一 ¥65/vial |
| 26 | 12-615F | Ham's F-12 Med w/ L-Gln | 20 | 买十送一 ¥65/vial |
| 27 | 12-726F | IMDM w/o L-Glut w/25 mM HEPES, 500ml | 20 | 买十送一 ¥65/vial |
| 28 | 12-169F | MEM, Alpha, w/o L-Gln | 20 | 买十送一 ¥65/vial |
| 29 | 12-662F | MEM Eagle EBSS w/ NEAA, Pyr, w/o L-Gln | 20 | 买十送一 ¥65/vial |
| 30 | 06-174G | MEM Eagle-EBSS w/ NEAA, L-Gln, w/o Ca++ | 20 | 买十送一 ¥65/vial |