Human Amyloid beta (aa1-42) Quantikine ELISA Kit阿尔茨海默科研用检测试剂盒
阿尔茨海默症（Alzheimer's Disease, AD），俗称老年痴呆症，其特征是在大脑神经细胞中存在老年斑（Senile Plaques，SPs） 和神经元纤维缠结（Neuro Fibrillary Tangles，NFT），老年斑的主要组成蛋白是 β- 淀粉样多肽（Amyloid Peptide，Aβ）40 和 42（43），Aβ42 比 Aβ40 更容易发生聚集。因此，蛋白样多肽的聚集开始于 Aβ42 而不是 Aβ40。出现了 Aβ42 而没有出现 Aβ40 是阿 尔茨海默症的早期特征；出现了 Aβ40，尤其是在成熟老年斑的中心部分出现则代表进入阿尔茨海默症的晚期阶段。

Human Amyloid beta (aa1-42) Quantikine ELISA Kit Summary

Assay Length 4.5 hours Sample Type & Volume Required Per Well Cell Culture Supernates (100 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL) Sensitivity 4.73 pg/mL Assay Range 7.8 - 500 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid) Specificity Natural human Amyloid beta (aa1-42). Cross-reactivity Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested. Interference No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Amyloid beta (aa1-42) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-42) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-42) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta
(aa1-42) showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-42).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	46.3	124	264	48.9	136	274
Standard Deviation	2.46	4.4	9.38	3.48	9.02	16.1
CV%	5.3	3.5	3.6	7.1	6.6	5.9

Recovery

The recovery of human Amyloid beta (aa1-42) spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	82-114
Cell Lysis Buffer (n=2)	92	90-94
Cerebrospinal Fluid (n=4)	107	95-120

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-42) were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.

Data Examples

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Human Amyloid beta aa 1-42 ELISA Standard Curve