Language
Sensitivity
1.0 pg/mL
Range
7.8-500 pg/mL
Reactivity
Mouse
Publications
151
Cat No : KE10003
Synonyms
Il 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta
| Product name | Mouse IL-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 102% | 91%-123% |
| Cell culture supernatants | 113% | 89%-126% |
| Cell lysates | 104% | 82%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 520.0 | 17.0 | 3.3 |
| 2 | 20 | 159.5 | 5.6 | 3.5 |
| 3 | 20 | 29.1 | 0.5 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 485.5 | 20.1 | 4.1 |
| 2 | 24 | 156.6 | 4.8 | 3.1 |
| 3 | 24 | 28.5 | 1.3 | 4.6 |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta |