Sensitivity
3.8 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Publications
195
Product Information
KE10007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-6. The assay recognizes mouse IL-6. An antibody specific for mouse IL-6 has been pre-coated onto the microwells. The mouse IL-6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-6 is added to detect the captured mouse IL-6 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sul furic acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Product name | Mouse IL-6 ELISA Kit |
Tests | 1 X 96 well plate |
Sample type | Serum, Plasma, Cell culture supernatants |
Assay type | Sandwich |
Sensitivity | 3.8 pg/mL |
Range | 15.6-1000 pg/mL |
Reactivity | Mouse |
Tested applications | Sandwich ELISA |
Cat No : KE10007
Synonyms
Il 6, Il6, IL-6, interleukin 6, Interleukin-6