Human Amyloid beta (aa1-40) Quantikine ELISA Kit，阿尔茨海默症科研检测试剂盒（仅限科研使用，不得用于临床） Assay Length 4.5 hours Sample Type & Volume Required Per Well Cell Culture Supernates (200 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL) Sensitivity 8.17 pg/mL Assay Range 15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid) Specificity Natural human Amyloid beta (aa1-40). Cross-reactivity Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested. Interference Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine^® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	58.9	264	556	56.3	247	518
Standard Deviation	4.23	5.76	11.3	4.31	22.4	30.5
CV%	7.2	2.2	2	7.7	9.1	5.9

Recovery

The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	99	95-105
Cell Lysis Buffer (n=2)	99	91-110
Cerebrospinal Fluid (n=4)	104	96-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.

Data Examples

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Human Amyloid beta (aa 1-40) ELISA Standard Curve

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Human Amyloid beta (aa 1-40) ELISA Standard Curve