C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cy7.
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146002
1 ml (reconstituted)
Description
GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.
Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Asialo-GM1
Formulation
Lyophilized powder. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application
FC - Quality tested Depletion - Reported in the literature, not verified in house
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.
This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12.
This product may contain other non-IgG subtypes.
Application References
(PubMed link indicates BioLegend citation)
Naiki M, et al. 1974. J. Immunol. 113:84.
Kasai M, et al. 1981. Nature. 291:334. (Depletion)
Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations
Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
Gauthier L, et al. 2019. Cell. 177:1701. PubMed
Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
Manangeeswaran M, et al. 2020. Front Immunol. 11:237. PubMed
Hu J, et al. 2015. J Virol. 89: 10383 - 10398. PubMed
Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
Li P, et al. 2020. Nat Commun. 3.504861111. PubMed
Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
RRID
AB_2562206 (BioLegend Cat. No. 146002)
Antigen Details
Structure
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution
NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons
Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References
1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.
Gene ID
NA
Pricing & Availability
Product Details
Antigen Details
Documentation
Reviews
Related Products
Related Pages & Pathways
Related FAQs
Other Formats
Concentration & Expiration Lookup
Certificate of Analysis
Pricing & Availability
Clone
Poly21460 (See other available formats)
Regulatory Status
RUO
Other Names
Ganglioside, GM1, ganglio-N-tetraosylceramide
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
11 publications
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cy7.
Compare all formats
Cat #
Size
Price
Quantity Check Availability
Save
146002
1 ml (reconstituted)
$485.00
Description
GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.
Product Details
Product Details
Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Asialo-GM1
Formulation
Lyophilized powder. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application
FC - Quality tested Depletion - Reported in the literature, not verified in house
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.
This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12.
This product may contain other non-IgG subtypes.
Application References
(PubMed link indicates BioLegend citation)
Naiki M, et al. 1974. J. Immunol. 113:84.
Kasai M, et al. 1981. Nature. 291:334. (Depletion)
Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations
Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
Gauthier L, et al. 2019. Cell. 177:1701. PubMed
Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
Manangeeswaran M, et al. 2020. Front Immunol. 11:237. PubMed
Hu J, et al. 2015. J Virol. 89: 10383 - 10398. PubMed
Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
Li P, et al. 2020. Nat Commun. 3.504861111. PubMed
Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
RRID
AB_2562206 (BioLegend Cat. No. 146002)
Antigen Details
Structure
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution
NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons
Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References
1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.
Language
NK细胞抑制剂抗体，Ultra-LEAF™ Purified