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| Parameter | HiLoad 16/600 Superdex 200 pg |
|---|---|
| Bed dimensions | 16 × 600 mm |
| Bed height | 600 mm |
| Includes | Dismantling tool, support screen, net ring (10 μm), O-ring, stop plugs, and instructions. |
| Bed volume | 120 |
| Column i.d. | 16 mm |
| Flow rate1 | 1.6 ml/min |
| Flow velocity | 10 cm/h - 50 cm/ |
| Order Information | HiLoad columns are prepacked XK columns. |
| Sample volume | < 5 ml |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack size | 1 × 120 mL |
HiLoad Superdex 200 pg prepacked columns are for high-resolution size exclusion chromatography of mAbs and other proteins from samples up to 5 mL or 13 mL.
- Prepacked format – for convenience and reproducibility; can be used with standalone pump or chromatography system.
- High resolution – for high purity.
- Scalable – separations can be scaled up using Superdex 200 prep grade resin.
- ÄKTA system compatible – Fittings (1/16”) for direct connection to ÄKTA systems and other chromatography systems.
Gel filtration column selectivity
This column is packed with Superdex 200 prep grade (pg) resin. This SEC resin combines excellent mechanical strength with high hydrophilicity, allowing high flow rates and minimal nonspecific interactions. Steep selectivity curves give excellent resolving power for proteins and other biomolecules in the molecular weight range: Mr ~ 10 000 to 600 000.
Successful use of Superdex 200 columns in research
These size exclusion columns are well suited to many applications, including:
- Separation of serum proteins
- Purification of human papillomavirus 16 L2E7 fusion protein
Other GE size exclusion chromatography columns
GE Healthcare offers a variety of Life Sciences solutions for gel filtration (size exclusion chromatography). Among these are columns for analysis, small-scale purification, or separation across a broader molecular weight range.