HiPrep Desalting columns with Sephadex G-25 resin

17508701

HiPrep 26/10 Desalting

53 mL

HiPrep 26/10 Desalting are convenient, easy-to-use, 53 mL columns prepacked with Sephadex G-25 Fine resin for desalting and buffer exchange.

• Provides reliable and reproducible protein desalting and buffer exchanges of samples up to 15 mL.
• Two to four columns can be connected in series for sample sizes from 30 to 60 mL.
• Replaces dialysis for desalting protein, which saves time and reduces labor.
• HiPrep format compatible with peristaltic pumps or chromatography systems such as ÄKTA systems.

Column for desalting and buffer exchange of proteins

HiPrep 26/10 Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction.

The exclusion limit is approximately Mr 5000. This ensures group separation of proteins/peptides larger than M_r 5000 from smaller molecules.

Successful use in published research

HiPrep 26/10 Desalting columns are used in a variety of research applications, including desalting in the purification process of myofilament proteins using a cleavable His6-tag.

Other GE products for buffer exchange or desalting

PD SpinTrap G-25 spin column for small sample quantities using a benchtop centrifuge – up to 180 µL sample volume PD MiniTrap G-25 gravity column for sample volumes up to 0.5 mL PD MidiTrap G-25 gravity column for sample volumes up to 1 mL PD-10 Desalting gravity column for sample volumes up to 2.5 mL HiTrap Desalting column for use with a syringe, a peristaltic pump, or a chromatography system Different types of Sephadex resins are also available as bulk resins

Parameter	HiPrep 26/10 Desalting
Bed dimensions	26 × 100 mm
Bed height	100 mm
Bed volume	53
Column i.d.	26 mm
Flow rate1	< 31 ml/min
Flow velocity	< 450 cm/h
Pressure max. (over the packed bed during operation)	1.5 bar [0.15 MPa] (22 psi)
Sample volume	<= 15 ml
Storage	4 to 30°C, 20% Ethanol
Pack size	53 mL