0.25% Trypsin-EDTA (1X), Phenol Red GIBCO胰酶25200056 促销 186元/100ml/瓶
GIBCO双抗15140122 276元/100ml/瓶。

描述

GIBCO^® 胰蛋白酶溶液广泛用于组织和单层细胞的解离。

包含:
• 2.5 g/L of 胰蛋白酶 (1:250)
• 0.38 g/L of EDTA•4Na
• 酚红
• 不含CaCl₂,MgCl₂•6H₂O, 和 MgSO₄•7H₂O的Hanks 平衡盐溶液 (HBSS)

性能和污染测试：
对胰蛋白酶进行生化检测，以检测在解离细胞过程中胰蛋白酶的活性和酶作用的专一性以及对细胞的影响。所有的胰蛋白酶溶液都经过了猪细小病毒和支原体实验检测。

Life Technologies 大家庭中的细胞培养产品。
Life Technologies公司已准备就绪，以满足您的细胞培养需要一个全面的选择，包括培养基，营养添加剂，盐溶液，缓冲液，化学物质，解离试剂和转染的 试剂。您可以依靠严格的质量控制和技术支持，以保证您的满意。仅供研究使用。 不用于任何动物或人类治疗或诊断使用。

详细说明

常用规格

Chelators:	EDTA
Phenol Red Indicator:	Phenol Red
Classification:	Animal Origin
Form:	Liquid
pH Range:	7.2 - 8.0
Osmolality:	270 - 320 mOsm/kg
Concentrated:	1 X
Product Size:	100 mL
Reagent Type:	Trypsin
Tests Performed:	In Vitro Bioassay
Shipping Condition:	Dry Ice
Regulatory Statement:	For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

内容和储存

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 18 months from date of manufacture

引文和参考文献 (3)

• Facilitated expansion of human embryonic stem cells by single-cell enzymatic dissociation.

作者

Ellerström C, Strehl R, Noaksson K, Hyllner J, Semb H

杂志

Stem Cells (2007) 25:1690-1696

产品用途

TrypLE select is better than trypsin for passage of human ES cells, which increases clone survival.

ID:

PN61410

• Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

作者

Zabzdyr Jennifer L; Lillard Sheri J;

杂志

Anal Chem (2002) 74:1857-1862

产品用途

Gibco (Invitrogen) 0.25% Trypsin-EDTA was used to remove Cho-K1 cells from cell culture flasks. Gibco (Invitrogen) penicillin/streptomycin was present in the Cho-K1 cell growth medium to prevent bacterial growth. Gibco F-12K (Kaighn's modification) is optimal for growing Cho-K1 cells.

ID:

PN65768

• Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.

作者

Benbow U; Butticè G; Nagase H; Kurkinen M;

杂志

J Biol Chem (1996) 271:10715-10737

产品用途

ProMMP-3 (the precursor of matrix metalloproteinase 3) and its mutant cDNA constructs were stably transfected into CHOK-1 cells using Lipofectin. Transfected cells were initially grown in glutamine-free Dulbecco's modified Eagles's medium containing dialyzed FBS and 25 µM methionine sulfoximine for 2 weeks. After colony formation, cells were split into 24-well plates and grown for additional 2 weeks in same medium with increasing concentrations of methionine sulfoximine (100-400 µM).

ID:

PN60108