T细胞活化/扩增试剂盒，T Cell Activation/Expansion Kit
The T Cell Activation/Expansion Kit was developed to activate and expand human T cells. The kit consists of Anti-Biotin MACSiBead Particles and biotinylated antibodies against human CD2, CD3, and CD28. Anti-Biotin MACSiBead Particles loaded with the biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture.

Downstream applications

Activated T cells can be used for any downstream processing, such as cytokine analysis ¹ or immunoprecipitation, and cells can also be transfected with high efficiency. The kit is suitable for the expansion of antigen-specific T cell lines and clones. It is also suitable for the activation of human CD3 ⁺ CD56 ⁺ NKT cells.

Figure 1

Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.

Stimulated sample	Unstimulated control
Stimulated sample	Unstimulated control