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QUANTI-Luc™
报告基因检测试剂
QUANTI-Luc™ is provided as packs of individually sealed pouches.
• rep-qlc1: 2 pouches of QUANTI-Luc™
• rep-qlc2: 5 pouches of QUANTI-Luc™
Each pouch contains everything needed to prepare 25 ml of reagent allowing the preparation of 500 wells of a 96-well plate.
QUANTI-Luc™ Procedure
1. Prepare QUANTI-Luc™ reagent by adding 25 ml water to the contents of one pouch.
2. Transfer samples, for instance 10 µl of media from cells containing the secreted luciferase to opaque 96-microwell plates suitable for your luminometer.
3. Set up the luminometer prior to addition of 50 µl QUANTI-Luc™ reagent to each well either manually or by automated injection. Measure luminosity in endpoint mode when using Lucia luciferase or in kinetic mode depending on the coelenterazine-luciferase used.
Recent articles using InvivoGen QUANTI-Luc™文献
- 2017 - Sci Rep., 7(1):2683.
RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling.
Fu B. et al. - 2016 - Nat Commun., 7:11492.
Behavioural traits propagate across generations via segregated iterative-somatic and gametic epigenetic mechanisms.
Mitchell E, Klein SL, Argyropoulos KV, Sharma A, Chan RB, Toth JG, Barboza L, Bavley C, Bortolozzi A, Chen Q, Liu B, Ingenito J, Mark W, Dudakov J, Gross S, Di Paolo G, Artigas F, van den Brink M, Toth M. - 2016 - Methods Mol Biol., 1393:105-11.
Exploiting secreted luciferases to monitor tumor progression in vivo.
Falcone L, Casucci M. - 2016 - J Neurosci., 36(5):1711-22.
Principles Governing DNA Methylation during Neuronal Lineage and Subtype Specification.
Sharma A, Klein SS, Barboza L, Lohdi N, Toth M. - 2016 - Chem Commun (Camb)., [Epub ahead of print]
Cellular delivery of enzyme-loaded DNA origami.
Ora A. et al.