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Human G-CSF ELISA KIT
粒细胞集落刺激因子G-CSF ELISA试剂盒 BMS2001INSTDescription: The human G-CSF Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human G-CSF. The human G-CSF Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Granulocyte colony stimulating factor (G-CSF) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukaemia cells.
The protein is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. The protein is highly conserved among species. G-CSF has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors and neutrophilic granulocytes as well as certain carcinoma cell lines. A soluble isoform of the G-CSF receptor has been described. Clinical use of G-CSF has been approved for several therapeutical applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and Aids.
G-CSF has further been shown to be a marker protein for different carcinomas such as bladder cancer.
参考文献：itations: Wojakowski,Wojciech; Tendera,Michal; Michalowska,Anna; Majka,Marcin; Kucia,Magdalena; Maslankiewicz,Katarzyna; Wyderka,Rafal; Ochala,Andrzej; Ratajczak,Mariusz Z.. Mobilization of CD34/CXCR4+, CD34/CD117+, c-met+ Stem Cells, and Mononuclear Cells Expressing Early Cardiac, Muscle, and Endothelial Markers Into Peripheral Blood in Patients With Acute Myocardial Infarction. Circulation 2004;110:3213-3220. (Link)
References: Rapoport,A.P.; Abboud,C.N.; DiPersio,J.F.. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF): receptor biology, signal transduction, and neutrophil activation. Blood Rev. 1992;6:43-57. (Link)
Reuss-Borst,M.A.; Muller,C.A.; Waller,H.D.. The possible role of G-CSF in the pathogenesis of Sweet's syndrome. Leuk.Lymphoma 1994;15:261-264. (Link)
Spiekermann,K.; Roesler,J.; Emmendoerffer,A.; Elsner,J.; Welte,K.. Functional features of neutrophils induced by G-CSF and GM-CSF treatment: differential effects and clinical implications. Leukemia 1997;11:466-478. (Link)
Wiedermann,F.J.; Mittermayr,M.; Hoffmann,G.; Schobersberger,W.. Recombinant granulocyte colony-stimulating factor (G-CSF) in infectious diseases: still a debate. Wien.Klin.Wochenschr. 2001;113:90-96. (Link)
Scarffe,J.H.; Kamthan,A.. Clinical studies of granulocyte colony stimulating factor (G-CSF). Cancer Surv. 1990;9:115-130. (Link)
Carr,R.; Modi,N.; Dore,C.. G-CSF and GM-CSF for treating or preventing neonatal infections. Cochrane.Database.Syst.Rev. 2003;CD003066(Link)
Tachibana,M.; Murai,M.. G-CSF production in human bladder cancer and its ability to promote autocrine growth: a review. Cytokines Cell Mol Ther. 1998;4:113-120. (Link)
Price,T.H.. Granulocyte transfusion in the G-CSF era. Int.J Hematol. 2002;76 Suppl 2:77-80. (Link)
Gabrilove,J.L.. Clinical applications of granulocyte colony stimulating factor (G-CSF). Growth Factors 1992;6:187-191. (Link)
Price,T.H.. The use of G-CSF in normal neutrophil donors. Ann Med Interne (Paris) 2000;151:115-122. (Link)
Cairo,M.S.. Review of G-CSF and GM-CSF. Effects on neonatal neutrophil kinetics. Am J Pediatr.Hematol.Oncol 1989;11:238-244. (Link)
Takano,H.; Ohtsuka,M.; Akazawa,H.; Toko,H.; Harada,M.; Hasegawa,H.; Nagai,T.; Komuro,I.. Pleiotropic effects of cytokines on acute myocardial infarction: G-CSF as a novel therapy for acute myocardial infarction. Curr.Pharm.Des 2003;9:1121-1127. (Link)
Tkatch,L.S.; Tweardy,D.J.. Human granulocyte colony-stimulating factor (G-CSF), the premier granulopoietin: biology, clinical utility, and receptor structure and function. Lymphokine Cytokine Res. 1993;12:477-488. (Link)
Tsuji,K.; Ebihara,Y.. Expression of G-CSF receptor on myeloid progenitors. Leuk.Lymphoma 2001;42:1351-1357. (Link)
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| Reactivity | Human |
|---|---|
| Sample Volume | 25 uL |
| Suitable Sample Types | cell culture supernatant, serum, plasma |
| Sensitivity | 11.0 pg/mL |
| Standard Curve Range | 39.1 - 2,500 pg/mL |
| Components | Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody to human G-CSF, Biotin-Conjugate (anti-G-CSF polyclonal antibody), Streptavidin-HRP and Sample Diluent lyophilized Aluminium pouch(es) with a human G-CSF Standard curve (coloured) Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20) Substrate Solution (tetramethyl-benzidine) Sample Diluent (Use when an external predilution of the samples is needed) Stop Solution (1M Phosphoric acid) Adhesive Plate Covers |
| Reported Applications | ELISA |
| Catalog Number | Product Description | Size |
| BMS2001INST | Human G-CSF Instant ELISA 128 tests | 128 tests |
| BMS2003INST | Human Iso-Insulin Instant ELISA 128 tests | 128 tests |
| BMS2006INST | Human TWEAK Instant ELISA 128 tests | 128 tests |
| BMS2007INST | Human BAFF Instant ELISA 128 tests | 128 tests |
| BMS2012INST | Human Caspase-3 Instant ELISA 128 tests | 128 tests |
| BMS201INST | Human sICAM-1 Instant ELISA 128 tests | 128 tests |
| BMS2020INST | Human Osteocalcin Instant ELISA 128 tests | 128 tests |
| BMS2021INST | Human Osteoprotegerin Instant ELISA 128 tests | 128 tests |
| BMS2029INST | Human MIP-1alpha Instant ELISA 128 tests | 128 tests |
| BMS202INST | Human TNF-beta Instant ELISA 128 tests | 128 tests |
| BMS2030INST | Human MIP-1beta Instant ELISA 128 tests | 128 tests |
| BMS2038INST | Human MPO Instant ELISA 128 tests | 128 tests |
| BMS2039INST | Human Leptin Instant ELISA 128 tests | 128 tests |
| BMS203INST | Human sTNF-R (60kDa) Instant ELISA 128 tests | 128 tests |
| BMS204/3INST | Human IL-8 Instant ELISA 128 tests | 128 tests |
| BMS205INST | Human sE-selectin Instant ELISA 128 tests | 128 tests |
| BMS2069INST | Human HGF Instant ELISA 128 tests | 128 tests |
| BMS206INST | Human sL-selectin Instant ELISA 128 tests | 128 tests |
| BMS2070INST | Human EGF Instant ELISA 128 tests | 128 tests |
| BMS207INST | Human sHER-2 Instant ELISA 128 tests | 128 tests |
| BMS209INST | Human sCD44std Instant ELISA 128 tests | 128 tests |
| BMS2105INST | Human sCD105 (Endoglin) Instant ELISA 128 tests | 128 tests |
| BMS210INST | Human sCD44var (v6) Instant ELISA 128 tests | 128 tests |
| BMS212INST | Human sIL-2R Instant ELISA 128 tests | 128 tests |
| BMS213INST | Human IL-6 Instant ELISA 128 tests | 128 tests |
| BMS214INST | Human sIL-6R Instant ELISA 128 tests | 128 tests |
| BMS215INST | Human IL-10 Instant ELISA 128 tests | 128 tests |
| BMS216INST | Human IFN-alpha Instant ELISA 128 tests | 128 tests |
| BMS218INST | Human sICAM-3 Instant ELISA 128 tests | 128 tests |
| BMS220INST | Human sCD44var (v5) Instant ELISA 128 tests | 128 tests |
| BMS221INST | Human IL-2 Instant ELISA 128 tests | 128 tests |
| BMS223INST | Human TNF-alpha Instant ELISA 128 tests | 128 tests |
| BMS224INST | Human IL-1 beta Instant ELISA 128 tests | 128 tests |
| BMS225INST | Human IL-4 Instant ELISA 128 tests | 128 tests |
| BMS229INST | Human sPECAM-1 Instant ELISA 128 tests | 128 tests |
| BMS231INST | Human IL-13 Instant ELISA 128 tests | 128 tests |
| BMS239INST | Human sCD40L Instant ELISA 128 tests | 128 tests |
| BMS240INST | Human sCD30 Instant ELISA 128 tests | 128 tests |
| BMS253INST | Human VE-Cadherin Instant ELISA 128 tests | 128 tests |
| BMS267INST | Human IL-18 Instant ELISA 128 tests | 128 tests |
| BMS278INST | Human IL-5 Instant ELISA 128 tests | 128 tests |
| BMS281INST | Human MCP-1 Instant ELISA 128 tests | 128 tests |
| BMS283INST | Human GM-CSF Instant ELISA 128 tests | 128 tests |
| BMS284INST | Human IP-10 Instant ELISA 128 tests | 128 tests |
| BMS285INST | Human MIG Instant ELISA 128 tests | 128 tests |
| BMS286INST | Human sCD27 Instant ELISA 128 tests | 128 tests |
| BMS287/2INST | Human RANTES Instant ELISA 128 tests | 128 tests |
| BMS288INST | Human C-reactive Protein Instant ELISA 128 tests | 128 tests |
| BMS291INST | Human sCD80 Instant ELISA 128 tests | 128 tests |
| BMS6002INST | Mouse IL-1beta Instant ELISA 128 tests | 128 tests |
| BMS6006INST | Mouse MCP-3 Instant ELISA 128 tests | 128 tests |
| BMS6007INST | Mouse MCP-5 Instant ELISA 128 tests | 128 tests |
| BMS6009INST | Mouse RANTES Instant ELISA 128 tests | 128 tests |
| BMS606INST | Mouse IFN-gamma Instant ELISA 128 tests | 128 tests |
| BMS607/2INST | Mouse TNF-alpha Instant ELISA 128 tests | 128 tests |
| BMS613INST | Mouse IL-4 Instant ELISA 128 tests | 128 tests |
| BMS614INST | Mouse IL-10 Instant ELISA 128 tests | 128 tests |
| BMS631INST | Rat MCP-1 Instant ELISA 128 tests | 128 tests |
| BMS640/3INST | Monkey IL-8 Instant ELISA 128 tests | 128 tests |
| BMS641INST | Monkey IL-6 Instant ELISA 128 tests | 128 tests |
| BMS642INST | Monkey IL-10 Instant ELISA 128 tests | 128 tests |
| BMS648INST | Monkey sICAM-1 Instant ELISA 128 tests | 128 tests |
| BMS649INST | monkey sE-selectin Instant ELISA 128 tests | 128 tests |
| BMS651INST | Monkey sPECAM-1 Instant ELISA 128 tests | 128 tests |
| BMS653INST | Monkey sTNF-RI Instant ELISA 128 tests | 128 tests |
