Language
Human R spondin 1/RSPO1 Protein
分子别名（Synonyms）R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
产品信息（Product Information）
表达区间及表达系统（Source） | Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus. |
分子量大小（Molecular Weight） | The protein has a predicted MW of 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result. |
纯度（Purity） | > 95% as determined by Bis-Tris PAGE |
内毒素（Endotoxin） | Less than 0.05EU per μg by the LAL method. |
制剂（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法（Reconstitution） | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储（Storage） | -20 to -80°C for 12 months as supplied from date of receipt. |
产品数据（Assay Data）
Bis-Tris PAGE
Human R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Cell Based Assay
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0 - 10.0 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).
ELISA Data
Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
ELISA Data
Immobilized Human R spondin 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4μg/ml determined by ELISA.
ELISA Data
Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human R spondin 1, His Tag with the EC50 of 4.4μg/ml determined by ELISA.
背景（Background）
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.