Language
Human Wnt Surrogate Protein
分子别名（Synonyms）Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
产品信息（Product Information）
表达区间及表达系统（Source） | Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc tag at the C-Terminus. |
分子量大小（Molecular Weight） | The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result. |
纯度（Purity） | > 95% as determined by Bis-Tris PAGE |
内毒素（Endotoxin） | Less than 1EU per μg by the LAL method. |
制剂（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法（Reconstitution） | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储（Storage） | -20 to -80°C for 12 months as supplied from date of receipt. |
产品数据（Assay Data）
Bis-Tris PAGE
Human Wnt Surrogate-Fc Fusion on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Cell Based Assay
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5.2 ng/mL.
背景（Background）
Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration. Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.