Language
Human Noggin Protein
分子别名（Synonyms）Noggin; NOG; SYM1; SYNS1
产品信息（Product Information）
表达区间及表达系统（Source） | Recombinant Human Noggin Protein is expressed from CHO without tag. |
分子量大小（Molecular Weight） | The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Bis-Tris PAGE result. |
纯度（Purity） | > 95% as determined by Bis-Tris PAGE |
内毒素（Endotoxin） | Less than 0.05EU per μg by the LAL method. |
制剂（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法（Reconstitution） | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储（Storage） | -20 to -80°C for 12 months as supplied from date of receipt. |
产品数据（Assay Data）
Bis-Tris PAGE
Human Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Cell Based Assay
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.
SEC-HPLC
The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.
背景（Background）
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events. Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.