HIGHLIGHTS

• Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
• Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
• Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion

DESCRIPTION

The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.