EliKine™ 小鼠胰高血糖素样肽-1 竞争法ELISA定量试剂盒
产品核心参数
- 货号:KTE7019
- 检测方法:竞争法ELISA(链霉亲和素-HRP系统)
- 样本兼容性:血清(浆)/组织匀浆/细胞裂解液/细胞培养上清
技术优势
- ⏩ 超高灵敏度:最低检测限8.23 pg/mL
- 特异性保障:无交叉反应干扰
- 宽动态范围:24.69-2000 pg/mL(R²≥0.99)
- 離 预混试剂:5×浓缩液体系,适配高通量检测
试剂盒组分
- 预包被抗小鼠GLP-1微孔板
- 冻干标准品(含梯度复溶方案)
- 生物素标记检测抗体(100×)
- 链霉亲和素-HRP复合物(100×)
- TMB底物/终止液系统
- 专用稀释缓冲液(5×)
关键实验参数
- 重复性:板内CV<8%,板间CV<12%
- 孵育体系:37℃避光孵育,总时长<4小时
- 数据稳定性:显色后30分钟内OD值波动<5%
质量控制方案
- 标准曲线强制包含6个有效浓度点
- 每板设置阳性对照孔和空白校准孔
- 推荐双孔检测模式(CV值≤15%)
物流保障
- 规格选择:
48 Tests ¥1,598 (现货次日达)
96 Tests ¥2,558 (现货次日达) - 冷链运输:蓝冰控温(2-8℃)
- 质保周期:12个月原厂验证
*本产品仅供科研使用,不可用于临床诊断。不同批次组分禁止混用,开盖后建议分装冻存。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。
EliKine™ Mouse Glucagon-like Peptide-1 Competitive ELISA Quantification Kit
Product Core Parameters
- Catalog No.:KTE7019
- Assay Principle:Competitive ELISA (Streptavidin-HRP System)
- Sample Compatibility:Serum/Plasma/Tissue Homogenate/Cell Lysate/Cell Culture Supernatant
Technical Advantages
- ⏩ Ultra-high Sensitivity:Lower detection limit: 8.23 pg/mL
- Specificity Assurance:No cross-reactivity interference
- Broad Dynamic Range:24.69-2000 pg/mL (R²≥0.99)
- 離 Pre-mixed Reagents:5× concentrated system for high-throughput detection
Kit Components
- Pre-coated anti-mouse GLP-1 microplate
- Lyophilized standard (with gradient reconstitution protocol)
- Biotin-labeled detection antibody (100×)
- Streptavidin-HRP conjugate (100×)
- TMB Substrate/Stop Solution System
- Specialized dilution buffer (5×)
Key Experimental Parameters
- Repeatability:Intra-assay CV<8%, Inter-assay CV<12%
- Incubation Protocol:37℃ protected from light, total duration <4 hours
- Data Stability:OD value fluctuation <5% within 30 minutes post-color development
Quality Control Protocol
- Standard curve must include 6 valid concentration points
- Positive control and blank calibration wells per plate
- Recommended duplicate testing mode (CV≤15%)
Logistics Assurance
- Specification Options:
48 Tests ¥1,598 (Next-day delivery available)
96 Tests ¥2,558 (Next-day delivery available) - Cold Chain Transport:Blue ice temperature control (2-8℃)
- Quality Warranty:12-month manufacturer validation
*For research use only. Not for diagnostic procedures. Do not mix components from different lots. Aliquot recommended after opening.
Note: Specifications are subject to change due to product optimization. Please contact ACROBiosystems for latest information.