Language
Full Name | Matrix Metalloproteinase 9 |
Synonym | MMP9;CLG4B;Gelatinase B;GELB;MANDP2;92kDa Type IV Collagenase;92 KDa Gelatinase; |
Assay Type | Sandwich |
Reactivity | Human |
Range | 0.15-10ng/mL |
Sensitivity | 0.09ng/mL |
Sample Type | Serum,plasma and other biological fluids |
Sample volume | 100μL |
UNIProt ID | P14780 |
Microplate | 96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle)：
本试产品采用双抗体夹心ELISA法。用抗Human MMP-9抗体包被于酶标板上，实验时样品（或标准品）中的Human MMP-9会与包被抗体结合。后依次加入生物素化的抗Human MMP-9抗体和HRP酶标记的亲和素，抗Human MMP-9抗体与结合在包被抗体上的Human MMP-9结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Human MMP-9浓度与OD450 OD值之间呈正比，通过绘制标准曲线计算出样品中Human MMP-9的浓度。
◤存储(Storage)：
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
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