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猪瘦素(LEP)酶联免疫吸附测定试剂盒

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上海恒斐生物科技有限公司
17317793691 021-61843644
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  • Brand  elabscience
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  • Main Business:ELISA试剂盒,抗体,生化试剂
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Last Updated 2025-08-09 00:20

猪瘦素(LEP)酶联免疫吸附测定试剂盒

本试剂盒采用双抗体夹心ELISA法。用抗猪LEP抗体包被于酶标板上,实验时样品或标准品中的猪LEP会与包被抗体结合,游离的成分被洗去。加入辣根过氧化物酶标记的抗猪LEP抗体。抗猪LEP抗体与结合在包被抗体上的猪LEP结合,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,LEP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LEP的浓度。
英文名称 Porcine LEP (Leptin) ELISA Kit
中文名称 猪瘦素(LEP)酶联免疫吸附测定试剂盒
货号 E-ELN-P0063c 种属 Porcine/猪
规格 96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
检测方法 双抗体夹心法
检测范围 0.781~50ng/mL 灵敏度 0.469ng/mL
中文名称 英文名称 规格 保存
ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8×12 / 8×6* 4℃/-20℃ #
冻干标准品 Reference Standard 2/1 支* 4℃/-20℃ #
标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20mL/12mL* 4℃
浓缩HRP酶结合物 Concentrated HRP Conjugate 1支 120μL/70μL* 4℃(避光)
酶结合物稀释液 HRP Conjugate Diluent 1瓶 10mL/6mL* 4℃
浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30mL/16mL* 4℃
底物溶液(TMB) Substrate Reagent 1瓶 10mL/6mL* 4℃(避光)
反应终止液 Stop Solution 1瓶 10mL/6mL* 4℃
封板覆膜 Plate Sealer 5/3 张*
产品说明书 Product Description 1 份
质检报告 Certificate of Analysis 1 份
特别说明:
*: [96T/48T](打开包装后请及时检查所有物品是否齐全完整)
#: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃.
Company Name 上海恒斐生物科技有限公司
Location
Business Scope ELISA试剂盒,抗体,生化试剂
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