Mouse Histone H4 ELISA Kit

Detect Range:0.156-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Sample Volume:100ul
Assay Time: 3-5h
Detection wavelength: 450 nm

Intended use
This immunoassay kit allows for the in vitro quantitative determination ofmouseHistone H4concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHistone H4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Histone H4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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