AssayPro—Human tPA Immuno-Chro

AssaySenseHuman tPA Immuno-Chromogenic Activity Assay Kit
组织纤溶酶原激活剂tPA酶免发色底物法活性试剂盒
Introduction
Tissue-type plasminogen activator (tPA) is a 68-kDa serine protease that converts the zymogen plasminogen into the active serine protease plasmin, which digests fibrin and induces the dissolution of fibrin clots (1). tPA is synthesized by endothelial cells in normal blood vessels and displays relatively high affinity for fibrin, suggesting that it functions predominately in physiological thrombolysis in vivo (2). A high level of tPA is a good prognostic marker for breast cancer (3). tPA may minimize the formation of metastasis by preventing tumor cell adherence at sites of trauma (4). On the other hand, gastrointestinal cancer is accompanied by a decrease in tPA (5).

Principle of Assay
The AssaySense Human tPA Immuno-Chromogenic Activity Assay Kit is developed to determine human tPA activity in plasma, serum and cell culture supernatants. The assay couples immunofunctional and indirect amidolytic assay. A polyclonal antibody specific for human tPA has been pre-coated onto a microplate and tPA is bound to the immobilized antibody. The assay measures the ability of tPA to catalyze a highly specific tPA substrate. The amount of tPA produced is quantitated using the highly specific tPA substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the tPA enzymatic activity.