Human alpha1-antitripsin ELISA

AssayMax Human alpha1-antitripsin ELISA Kit
α1抗胰蛋白酶α1-AT酶联免疫试剂盒
Introduction
Alpha-1 antitrypsin (A1AT) is a protein that protects the lungs. The liver usually makes the protein, and releases it into the bloodstream. A1AT is a major protease inhibitor that controls tissue degradation. A reduction of A1AT levels can cause a change in collagen metabolism (1). A1AT inhibits neutrophil elastase release into the lungs during inflammatory states (2). A1AT deficiency is an uncommon genetic disease (3) that can lead to emphysema (4), hepatitis, cirrhosis (5), and chronic obstructive pulmonary disease (COPD) (6).

Principal of the Assay
The AssayMax Human A1AT ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human A1AT in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human A1AT in less than 3 hours. A polyclonal antibody specific for human A1AT has been pre-coated onto a 96-well microplate with removable strips. A1AT in standards and samples is competed with a biotinylated A1AT sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.