Language
The Human IgG4 Uncoated ELISA Kit contains pre-matched antibody pair, platess and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgG4. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Human IgG4, also known as human immunoglobulin G4, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the least abundant subclass of IgG in the blood and plays a minor role in defense against pathogens. IgG4 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG4 is also involved in other immune responses, such as immune tolerance and suppression of inflammation. Human IgG4 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.
引用文献
[1] Radosavljević J, Apostolović D, Mihailović J, Atanasković-Marković M, Burazer L, van Hage M, Ćirković Veličković T. Digestomics of Cow's Milk: Short Digestion-Resistant Peptides of Casein Form Functional Complexes by Aggregation. Foods. 2020 Oct 30;9(11):1576. doi: 10.3390/foods9111576. PMID: 33143174; PMCID: PMC7692894.
[2] Khil'chenko S, Boch K, van Beek N, Vorobyev A, Zillikens D, Schmidt E, Ludwig RJ. Alterations of Total Serum Immunoglobulin Concentrations in Pemphigus and Pemphigoid: Selected IgG2 Deficiency in Bullous Pemphigoid. Front Med (Lausanne). 2020 Sep 2;7:472. doi: 10.3389/fmed.2020.00472. PMID: 33015084; PMCID: PMC7493804.
