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大鼠糖原合成酶激酶3β(GSK3B)ELISA kit/ R
- 产品类型: ELISA Kit
- 产品名称:
大鼠糖原合成酶激酶3β(GSK3B)ELISA kit
- 英文名称:
Rat Glycogen synthase kinase-3 beta(GSK3B) ELISA kit
- 货号: CSB-EL009963RA
- 别名: , GSK3beta isoform
- 规格: 96T
- 中文价格: 3600
- 种属: Rat
- 待测物名称: glycogen synthase kinase 3 beta
- 缩写: GSK3B
- 蛋白功能1: Biosynthesis/Metabolism
- 蛋白功能2: glyconeogenesis and glycometabolism
- 蛋白功能3: Carbohydrate metabolism
- 检测范围: 0.156 ng/ml-10 ng/ml
- 灵敏度: 0.039 ng/ml
- 反应时间: 1-5h
- 所需样本体积: 50-100ul
- 检测波长: 450 nm
- 标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml | OD1 | OD2 | Average | Corrected | | 10 | 2.241 | 2.356 | 2.299 | 2.147 | | 5 | 1.544 | 1.648 | 1.596 | 1.444 | | 2.5 | 1.022 | 0.992 | 1.007 | 0.855 | | 1.25 | 0.661 | 0.654 | 0.658 | 0.506 | | 0.625 | 0.423 | 0.432 | 0.428 | 0.276 | | 0.312 | 0.307 | 0.315 | 0.311 | 0.159 | | 0.156 | 0.228 | 0.224 | 0.226 | 0.074 | | 0 | 0.151 | 0.152 | 0.152 | | | |
- 精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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- 线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat GSK3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 92 | |
Range % | 86-96 | |
1:2 | Average % | 102 | |
Range % | 97-104 | |
1:4 | Average % | 95 | |
Range % | 89-99 | |
1:8 | Average % | 97 | |
Range % | 92-104 | |
- 回收率:
The recovery of rat GSK3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 94 | 89-97 | |
EDTA plasma (n=4) | 98 | 92-102 | |
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- 研究领域: Metabolism
- 引用文献:
1. , Zhang Q.et al,Toxicology.,2016,347-349:40-6. |
2. , Rana A. et al,Clin Exp Pharmacol Physiol,2015,19 |
3. , Khan A.et al,Neuroscience.,287:66-77 |
Company Name |
北京爱特蒙科技有限公司 |
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