AssayPro—Rat Transferrin ELISA

AssayMax Rat Transferrin ELISA Kit
大鼠转铁蛋白TRF酶联免疫试剂盒
Introduction
Transferrin is a plasma protein that transports iron through the blood to the liver, spleen, and bone marrow. Low transferrin level in plasma could associate with anemia (1) and chronic liver disease (2). On the other hand, high plasma transferrin level could indicate iron deficiency anemia (3).

Principle of the Assay
The AssayMax Rat Transferrin ELISA kit is designed for detection of rat transferrin in urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures transferrin in less than 4 hours. A polyclonal antibody specific for rat transferrin has been pre-coated onto a microplate. Transferrin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for transferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.