Rat Ceruloplasmin ELISA Kit（大鼠

AssayMax Rat Ceruloplasmin ELISA Kit
大鼠铜蓝蛋白CP酶联免疫试剂盒
Introduction
Ceruloplasmin is an abundant α2-serum glycoprotein that contains 95% of the copper found in the plasma of vertebrate species (1). Ceruloplasmin is a copper-binding protein that normally removes iron from cells by its ferroxidase activity. Ceruloplasmin concentration on average is 14.6 (± 4.0) mg/dl (2). Low levels of ceruloplasmin lead to the abnormal deposition of iron in cells, including those of the pancreas, liver, retina, and the basal ganglia region of the brain. Some diseases associated with ceruloplasmin are Wilson's disease (3), Hemochromatosis (4), Menkes disease (5), and Aceroluplasminemia (1).

Principle of the Assay
The AssayMax Rat Ceruloplasmin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of rat ceruloplasmin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures rat ceruloplasmin in less than 3 hours. A polyclonal antibody specific for rat ceruloplasmin has been pre-coated onto a 96-well microplate with removable strips. Ceruloplasmin in standards and samples is competed by a biotinylated ceruloplasmin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.