ATM (Q2442P)

ATM (Q2442P)

Catalog  Number: TD26142 
Gene  Symbol:  ATM
Description: Anti-ATM (Q2442P) Mouse Monoclonal Antibody
Background: Ataxia telangiectasia  mutated (ATM) is a  serine/threonine  protein  kinase  that is recruited  and  activated  by  DNA double-strand breaks.  It  phosphorylates several key  proteins that initiate  activation  of  the  DNA damage checkpoint,  leading  to  cell  cycle  arrest,  DNA repair  or  apoptosis. Activity  of ATM protein  is under tight  control,  and  mutation  of ATM can cause  disease such  as Ataxia  telangiectasia (AT) and  cancers.
Immunogen: A synthetic  peptide  from  the internal region  of ATM which  includes  the mutation  of  Q2442P,  huma n  origin. 
Tested applications: ELISA,  WB,  IHC 
Recommended dilutions: ELISA: 1:1000-1:5000    WB:1:100-1:1000    IHC:1:50-1:500 
Concentration:  1  mg/ml 
Host: Mouse 
Clonality:  Monoclonal 
Purity: Purified  from ascites 
Format: Liquid
Preservative: no
Constituents: PBS  (without Mg2+   and  Ca2+), pH 7.4, 150  mM  NaCl,  50% glycerol
Species Reactivity: Recognizes ATM (Q2442P), but  not  wild  type ATM protein  from vertebrates. 
Storage Conditions:  Store  at -20°C. Avoid freeze  / thaw  cycles.
Western blot:

Western blot  analysis of  recombinant ATM (Q2442P) and  wildtype  proteins.  Purified His-tagged ATM (Q2442P) protein  (amino  acids 2400-3056,  lane  2) and  corresponding  wild  type protein  (lane  1) were blotted  with  anti-ATM (Q2442P)       mouse  monoclonal  antibody  (Cat. #TD-26142).