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TP53(R175H)
| Cat. #: TD-26072 |
| Gene Symbol: TP53(R175H) |
| Description: Anti-TP53(R175H) Mouse Monoclonal Antibody |
| Background: TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on. |
| Immunogen: A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-TP53(R175H) antibody recognizes TP53(R175H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot:  Western blot analysis of recombinant TP53(R175H) and wild type proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wild type protein(Cat.#) were blotted with Anti-TP53(R175H) monoclonal antibody(Cat.# TD-26072). Immunofluorescence: Immunofluorescence of cells expressing TP53 proteins with anti-TP53(R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53(R175H) plasmid (right column), then fixed and stained with anti- TP53(R175H) monoclonal antibody (Cat. # TD-26072). |
