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Ras(G13A)
| Cat. #: TD-26191 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti-HRAS(G13A) Mouse Monoclonal Antibody |
| Background: Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane. |
| Immunogen: A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G13A mutant, but not wild type H-RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:  Western blot analysis of recombinant HRAS(G13A) and wild type proteins. Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-HRAS(G13A) monoclonal antibody (Cat. #TD-26191). Immunofluorescence: Immunofluorescence of cells expressing Ras proteins with Anti-HRAS(G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRAS(G13A) plasmid (right column), then fixed and stained with Anti-HRAS(G13A) monoclonal antibody (Cat. #TD-26191). |
