ATP定量分析试剂盒

RayBio®ATP定量分析是一种快速、高敏和简单的方法，利用萤火虫荧光素酶的ATP依赖性，通过吸光度测定溶液中的ATP含量。

该试剂盒可用于在各种培养基（细胞培养，PBS，水等）中评估细胞活力，细胞毒性和/或代谢活性。

货号：NA-ATP

规格：96T

灵敏度：0.064 pmol

适用样本：各种培养基（细胞培养，PBS，水等）    

产品特性：高敏，兼容各种液体，方法简单

Kit Components

• Dilution Buffer
• Assay Buffer
• Lysis Buffer
• ATP Standard
• Luciferase
• DTT
• D-Luciferin
• 96-Well White-Walled Microplate
• Plate Seal

Additional Materials Required

• Sterile Phosphate Buffered Saline (PBS)
• Vortex Mixer
• Tabletop centrifuge
• Pipettes and nuclease-free pipette tips (barrier tips recommended)
• Nuclease-free 15 mL conical tubes and 1.5 mL microcentrifuge tubes
• Plate reader capable of measuring luminosity

Assay Procedure Summary

1. Prepare all reagents, samples and standards as instructed.
2. Add standard or sample to each well of the microplate.
3. Add Master Mix to each well and mix.
4. Protect from light and allow to equilibrate to room temperature for 10 minutes.
5. Measure luminosity.

Storage/Stability

Remove D-luciferin vials from kit and store at or below -70°C protected from light. The remaining items of the kit can be stored together at -20°C or separately according to indicated storage temperatures. Kit is stable for up to four months.