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血凝块液化柱

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  • 138.89/

上海莱枫生物科技有限公司
18012236082 021-64810180
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  • Brand  lifefeng
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  • Main Business:PCR,核酸纯化,核酸电泳
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Last Updated 2025-08-12 12:39

血凝块液化柱

血凝块液化柱含刚性细孔径筛网结构,通过离心的方式将血凝块剪切、分散为不堵塞枪头的泥状物,方便了后续的DNA提取工作,能明显提高DNA的产量和纯度。
最大柱体积为900 μl,更大体积样品可重复过柱。

产品使用说明书:血凝块液化柱
更多产品信息请关注:www.lifefeng.com

关于从血凝块中提取DNA
非抗凝血液中血纤维网罗红细胞和绝大部分白细胞形成粘稠、致密的血凝块。分散血凝块是从中有效提取DNA的关键;为此我们特别推荐血凝块液化柱,简单的离心过滤步骤即可充分分散血凝块。
非抗凝血无外加的二价盐离子螯合剂和肝素等DNase抑制剂,其中的DNA容易发生凋亡和降解。建议使用DK603或DK604进一步处理液化的血凝。DK603和DK604预处理步骤使用Buffer MG-A促使>5 kb 的DNA形成容易沉淀的凝集物,只有少量降解的小片段DNA共沉淀被回收。去除降解的小片段DNA有利于基于PCR原理的后续实验。


使用血凝块液化柱处理非抗凝血,后续使用DK603提取DNA
M:DL15,000 0.8%agarose
1和2为同一份样品;
3和4为同一份样品;
1和3未使用血凝块液化柱
2和4使用血凝块液化柱
Company Name 上海莱枫生物科技有限公司
Location
Business Scope PCR,核酸纯化,核酸电泳
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