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属性 用途 sufficient for 1600 cm2 sufficient for 20 miniblot(s) manufacturer/tradename Calbiochem® 储存条件 OK to freeze protect from light detection method chemiluminescent colorimetric fluorometric 运输 wet ice 储存温度 2-8°C 相关类别 Immunodetection Reagents & Substrates 说明 一般描述 SignalBoost Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies. It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required. • Increases signal: minimizes background resulting in high signal to noise ratios • Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems • Convenient: ready-to-use format, no dilution necessary SignalBoost enhances antigen-antibody reactions, yielding signals several times to several tens of times higher than those obtained with conventional methods. It has been verified that enhancement is particularly marked for relatively inactive antibodies. Compositions optimized for primary and secondary antibodies respectively minimize background and yield high S/N ratios. It can be used for various assays involving antigen-antibody reactions, such as immunoblotting, dot blotting and ELISA. Components do not affect the activity of enzymes used for labeling (peroxidase, alkaline phosphatase, etc.), allowing use in assays using any of these enzymes. It can also be used for detection of both fluorescence and coloration. For convenience, SignalBoost is supplied in a ready-to-use form, is at an appropriate concentration for use, and does not need to be diluted. Simply dilute antibodies with this product instead of conventional diluents to use. It does not lengthen the time required for the assay. 包装 1 kit in Plastic ampoule 组分 Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol. 警告 Toxicity: Standard Handling (A) 原理 SignalBoost Immunoreaction Enhancer Solution is a reactive reagent for use in immunoassays, and was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as immunoblotting and ELISA (enzyme-linked immunosorbent assay). It can be used in a range of immunoassays. 外形 Format: Liquid, Antibody Diluent 储存及稳定性 Upon receipt, store the entire contents of the kit at 4°C. Protect from light. Following initial use, this product is stable for up to 1 year at 4°C. 法律信息 CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany 安全信息 象形图 Exclamation markEnvironment GHS07,GHS09 警示用语： Warning 危险声明 H317 - H411 预防措施声明 P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 危险分类 Aquatic Chronic 2 - Skin Sens. 1 储存分类代码 10 - Combustible liquids WGK WGK 3
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