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Ni亲和介质 满1L送IPAD mini4

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  • 3472.22/

杭州艾珞生物科技有限公司
13758257082 0571-88035953
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  • Brand  Arobio
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Last Updated 2025-08-12 13:15

Ni亲和介质 满1L送IPAD mini4

1. 产品简介

琼脂糖亲和介质(Ni)属于一类金属螯合介质,它是将亚氨基二乙酸(IDA)或次氮基三乙酸(NTA)键合在高流速琼脂糖微球上,并螯合金属离子Ni2+而形成的一种亲和层析介质。该亲和介质不仅具有吸附容量大、选择性好、通过性强、易于再生、成本低等优点,还有利于保持产品的生物活性和提高产品的收率,从而广泛用于生物制药和生物工程下游蛋白质、核酸及多肽的分离纯化,尤其是组氨酸标记蛋白质的高效制备。 2. 产品特性
参数 指标
基质 4%交联琼脂糖凝胶
配基 IDA(或NTA)
形状 球形
介质平均粒径 90 μm (45~165)
金属离子载量 15-120 μmol Ni2+/ml(可调)
动态载量 20~30 mg LDH/ml wet gel
最高流速(25℃) 500 cm/h
最高耐压 0.3 MPa (3 bar)
pH稳定性 3~13(长时间);2~14(短时间)
保存 4~8 oC(20%乙醇)
3. 应用 采用琼脂糖亲和介质(Ni)对组氨酸标记蛋白质进行分离纯化的过程通常包括装柱、平衡、进料、淋洗、洗脱、再生等步骤。
Company Name 杭州艾珞生物科技有限公司
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