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金属螯合介质(NTA)

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江苏苏青生物技术有限公司
15601694133 00000000
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  • Brand  Suqingbio
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Last Updated 2025-08-12 13:18

金属螯合介质(NTA)


金属螯合介质(NTA),属于快样亲和介质,有四个金属离子螯合位点,相比其他仅有三个金属离子螯合位点(IDA)的金属螯合纯化介质而言,能够更紧密地结合金属离子。多出的 一个螯合位点能够有效避免金属离子脱落,因此具有更强的结合能力,相比那些使用其他金属螯合纯化体系的方法,能够获得更大的结合量和更纯的蛋白制备产物。Ni-NTA纯化介质可以纯化任何表达系统(原核,酵母,昆虫细胞和哺乳动物细胞等表达系统)表达的天然或变性的His-标签蛋白。结合在介质上的蛋白可以通过低pH缓冲液或者咪唑进行洗脱,保证his-标签蛋白活性。
1.2 产品性能特点
  • 低非特异性结合,低金属离子脱落;
  • 高亲和能力及高载量
  • 高效、快速一步纯化His标签蛋白;纯度高达90%以上
  • 耐受还原剂,可在天然或变性条件下进行蛋白纯化
  • 可选择不同的金属离子进行蛋白纯化
  • 适用于注射器,蠕动泵或层析系统(如AKTA)
  • 提供预装柱及散装两种包装形式自有选择

Company Name 江苏苏青生物技术有限公司
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