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蛋白A亲和介质

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国家生化工程技术研究中心(北京)
010-82544957
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Last Updated 2025-08-12 13:21

蛋白A亲和介质

蛋白A纯化分离介质

1、产品简介

Protein A能特异性地与抗体的Fc区结合,所以Protein A亲和介质可用于抗体(单抗和多抗)的分离纯化,经过一步亲和层析,即可从腹水、血清和培养液等样品中得到高纯度的抗体。Protein A QZT 4FF亲和介质以自制的琼脂糖凝胶为基质,以重组Protein A为配基,具有很高的物理化学稳定性,配基不易脱落,寿命长,使用方便,应用广泛。


2、产品特性

参数 指标
基质 4%交联琼脂糖凝胶
配基 r-Protein A
形状 球形
介质平均粒径 90 μm (45-165)
配基密度 6mg ProteinA/ml wet gel
动态载量 30-40mg rabbitIgG/ml wet gel
最高流速(25℃) 450 cm/h
推荐流速 <150 cm/h
最高耐压 0.3 MPa (3 bar)
pH稳定性 3~10(长时间);2~11(短时间)
保存 4~8 oC(20%乙醇)

3、应用

Protein A QZT 4FF广泛用于各种抗体的分离纯化。层析操作通常包括装柱、平衡、进料、淋洗、洗脱、再生等步骤。

Company Name 国家生化工程技术研究中心(北京)
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