293T细胞系

293T细胞293T人胚肾细胞

Cell line name HEK293T

Synonyms Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo

Accession CVCL_0063

Resource Identification Initiative To cite this cell line use: HEK293T (RRID:CVCL_0063)

Comments Part of: ENCODE project common cell types; tier 3.

Part of: MD Anderson Cell Lines Project.

Virology: Highly susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).

Doubling time: ~24-30 hours (DSMZ=ACC-635).

Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.

Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.

Transformant: NCBI_TaxID; 28285; Adenovirus 5.

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).

Omics: Deep proteome analysis.

Omics: Genome sequenced.

Omics: miRNA expression profiling.

Omics: Mitochondrial proteome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Transcriptome analysis by RNAseq.

Misspelling: HECK293T; Note=Occasionally.

Misspelling: HEK 239T; Note=Occasionally.

Misspelling: HEK239T; Note=Occasionally.

Misspelling: HEK-393T; Note=Occasionally.

Misspelling: HEK393T; Note=Occasionally.

Misspelling: 239T; Note=Occasionally.

Misspelling: 393T; Note=Occasionally.

Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.

PubMed=3031469; DOI=10.1128/mcb.7.1.379-387.1987; PMCID=PMC365079

DuBridge R.B., Tang P., Hsia H.-C., Leong P.-M., Miller J.H., Calos M.P.

Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.

Mol. Cell. Biol. 7:379-387(1987)

PubMed=15900046; DOI=10.1093/jnci/dji133

Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J.-i., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.

p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.

J. Natl. Cancer Inst. 97:765-777(2005)

PubMed=25182477; DOI=10.1038/ncomms5767; PMCID=PMC4166678

Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A., Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F., Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.

Nat. Commun. 5:4767.1-4767.12(2014)

PubMed=26694163; DOI=10.1371/journal.pone.0144924; PMCID=PMC4692263

Janiszewska J., Szaumkessel M., Kostrzewska-Poczekaj M., Bednarek K., Paczkowska J., Jackowska J., Grenman R., Szyfter K., Wierzbicka M., Giefing M., Jarmuz-Szymczak M.

Global miRNA expression profiling identifies miR-1290 as novel potential oncomiR in laryngeal carcinoma.

PLoS ONE 10:E0144924-E0144924(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076

Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

PubMed=28601559; DOI=10.1016/j.cels.2017.05.009; PMCID=PMC5493283

Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.

An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.

Cell Syst. 4:587-599.e4(2017)

DOI=10.13005/bpj/1414

Yuan J., Xu W.W.-Y., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.

The scattered twelve tribes of HEK293.

Biomed. Pharmacol. J. 11:621-623(2018)

PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893

Himmelsbach K., Hildt E.

Identification of various cell culture models for the study of Zika virus.

World J. Virol. 7:10-20(2018)

PubMed=34800366; DOI=10.1016/j.cmet.2021.11.001; PMCID=PMC8664129

Morgenstern M., Peikert C.D., Lubbert P., Suppanz I., Klemm C., Alka O., Steiert C., Naumenko N., Schendzielorz A., Melchionda L., Muhlhauser W.W.D., Knapp B., Busch J.D., Stiller S.B., Dannenmaier S., Lindau C., Licheva M., Eickhorst C., Galbusera R., Zerbes R.M., Ryan M.T., Kraft C., Kozjak-Pavlovic V., Drepper F., Dennerlein S., Oeljeklaus S., Pfanner N., Wiedemann N., Warscheid B.

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Cell Metab. 33:2464-2483.e18(2021)