| Organism | Homo sapiens, human |
|---|---|
| Tissue | peripheral blood |
| Cell Type | T lymphoblast |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | acute lymphoblastic leukemia |
| Age | 4 years juvenile |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 to 3 X 105 and 1 to 2 X 106 viable cells/mL. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 7.5% DMSO. Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
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