ESGRO® (LIF)

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Specific Activity: ESGRO® is assessed both on ES {embryonic stem} cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 10⁶ units is equivalent to approximately 10 µg of pure protein, and is sufficient to treat 1L of ES cell culture media.

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10⁸ units/mg

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

At the recommended concentration 10⁷ units of ESGRO® is sufficient for 10.0 L of tissue culture media and 10⁶ units of ESGRO® is sufficient for 1.0 L of tissue culture media.

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