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AcceGen小鼠胚胎成纤维细胞NIH-3T3细胞(ABC-TC0828)
产品简介：
产品英文名称：NIH-3T3；
货号：ABC-TC0828；
物种：鼠；
产品类型：小鼠胚胎细胞系；
描述：小鼠瑞士NIH胚胎。由NIH瑞士小鼠胚胎建立。这些细胞对肉瘤病毒病灶形成和白血病病毒传播敏感。细胞现在已经失去了接触抑制；
生长特征：成纤维细胞；
推荐培养基和补充剂：90%DMEM，2mM谷氨酰胺，10%小牛血清（CS）；
生物安全等级：一级；
运输方式：干冰运输；
储存方式：液氮储存；
应用：仅供研究使用。
细胞培养：
1、培养前准备
（1）培养器具：推荐使用25 cm2培养瓶或60 mm培养皿（或更小）。
（2）培养基：准备适合培养AcceGen小鼠胚胎成纤维细胞NIH-3T3细胞的培养基，建议用量5 mL（或更少）。
2、细胞解冻
将包装中的冻存管取出后立即放在不高于37℃的水中，并在2分钟内摇晃解冻。
3、细胞复苏
（1）取10ml培养基置于离心管中，用70%酒精或阳离子洗涤剂渗透纱布消毒冻存管，用消毒纱布包裹管身，拧开管盖后，将细胞悬浮液转移到离心管中；
（2）1000 rpm离心3分钟，弃掉上清液；
（3）将细胞重新悬浮在5ml上述培养基中（不清洗细胞）。在25 cm2的培养瓶或60 mm的培养皿中进行培养。
4、细胞传代
在传代之前，确保细胞增殖，因为过度稀释可能导致细胞死亡，尤其是血细胞和淋巴细胞。
5、细胞冻存
待AcceGen小鼠胚胎成纤维细胞NIH-3T3细胞生长状态良好时，尽早进行细胞冻存。
NIH-3T3 | ||||
|---|---|---|---|---|
| Product Name | NIH-3T3 | |||
| Product Code | NIH/3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3 | |||
| Cat.No | ABC-TC0828 | Species | Mouse | |
| Size/Quantity | 1 vial | Biosafety Level | 1 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | NIH-3T3 cells, also recognized as Swiss 3T3, trace their origin to a mouse NIH/Swiss embryo and represent a fibroblast cell line characterized by distinctive attributes. Demonstrating heightened sensitivity to sarcoma virus focus formation and leukemia virus propagation, this multiclonal lineage manifests discernible clonal aberrations, housing a subclonal contingent predisposed to cellular transformation. Marked by perpetual proliferation in vitro and endowed with immortality, NIH-3T3 cells boast a doubling time of approximately 20 hours. When dispersed randomly on a chitosan surface, these cells assume a spindle-like morphology, while alignment on an OCMCS surface induces an elongated form. Despite an initial penchant for extreme contact inhibition, this hallmark has progressively waned within the cell line over successive passages.
Why choose NIH-3T3 from AcceGen? NIH-3T3 cells from AcceGen are characterized by high viability and quality. Rigorous quality control measures, including a PCR-based assay, guarantee the absence of mycoplasma. Daily monitoring ensures the cells remain free from bacteria, fungi, and yeast contamination. Additionally, the cell line’s authenticity is confirmed through STR analysis for precise cell identification. | |||
| Disease | Normal | |||
| Source Organ | Embryo | |||
| Recommended Medium And Supplement | DMEM + 10% FBS | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | FOR RESEARCH USE ONLY The NIH-3T3 cell line, a versatile fibroblast cell type, finds wide applications in DNA transfection studies and scientific research. Renowned for its spontaneous immortalization of mouse embryo cells, the 3T3 cell line serves as a valuable recipient in experiments involving the transfer of DNA from tumors. This application facilitates the discernment of transforming oncogenes, positioning NIH-3T3 cells as an indispensable tool for delving into the intricate molecular mechanisms governing cellular transformation and oncogenic processes in rigorous scientific investigations. | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Fibroblast | |||
| Growth Mode | Adherent | |||
| Product Type | Mouse Embryo Cell Lines | |||
产品信息：
| 货号 | 产品名称 | 规格 |
| ABC-TC0828 | NIH-3T3 | 1 vial |
